ABSTRACT
In late 2019 a new virus reported in Wuhan, China, identified as SARS-CoV-2, rapidly challenging the healthcare system worldwide. The need for rapid, timely and accurate detection was critical to the prevention of community outbreaks of the virus. However, the high global demand for reagents during the years 2020 and 2021 generated a bottleneck in kits used for detection, significantly affecting developing countries and lagging their ability to diagnose and control the virus in the population. The difficulty in importing reagents, high costs and limited public access to the SARS-CoV-2 detection test led to the search for alternative methods. In this framework, different commercial nucleic acid extraction methodologies were evaluated and compared against heat shock as an alternative method for SARS-CoV-2 detection by RT-PCR to determine the diagnostic yield and its possible low cost compared to other methodologies. Nasopharyngeal samples were used where the diagnostic efficiency of the alternative method was 70 to 73%. The evaluation of the discriminatory efficacy of the technique took the sensitivity and specificity to establish its cut-off point, being 0.73 to 0.817, which allows discrimination between COVID-19 positives and negatives;as for the diagnostic effectiveness expressed as, the proportion of subjects correctly classified is between 80 and 84%. On the other hand, in terms of the costs necessary to carry out the detection, the alternative method is more economical and accessible compared to the commercial methods available in this comparison and evaluation, being possible its implementation in developing countries with high infection rates, allowing access to the diagnostic test with a reliable and low-cost method. © 2022 by the authors.